As we know that current era is the time of biotechnology and especially I would say rDNA Technology. In this context there is a key need to understand the concept of rDNA Technology as such. Keeping in mind the basic need of students, this book is designed. All the chapters are in very simple language. The chapter 1 deals with key DNA manipulating enzymes followed by chapter 2 which deals with the most important part of rDNA and that is vectors. The llabeling of the nucleic acids have been described in chapter 3 followed by chapter 4 and 5 which overall deals with cDNA/genomic libraries, PCR and applications. Sequencing of the nucleic acid has been described in chapter 6. Nevertheless, to gain insight into the applications of rDNA Technology, chapter 7 has been designed. The whole content is simply presented, and orderly arranged. I am fully sure that the students will gain a lot from this book and I encourage them to read and suggest the modifications in the coming time.
Contents :
1. rDNA Technology: DNA Manipulating Enzymes
2. Cloning Vectors
3. Labeling of Nucleic Acids
4. Polymerase Chain Reaction (PCR) Other Blotting Techniques, Introduction to Genomics, Proteomics and Bioinformatics
5. Transformation, cDNA and Genomic Libraries
6. Sequencing of Nucleic Acid
7. Applications of rDNA Technology
8. Changing Genes: Site Directed Mutagenesis
